raven19

Hello, after analyzing exomes with AnnotSV I have some interesting duplications and deletions to validate. I only now how to validate deletions with qPCR and Sybr Green; However this is not the best approach for duplications. These duplicated/deleted genes also are not in ready MRC Holland MLPA probe sets. Which method is best for validating duplications and deletions of some exons in genes? Thank you!