Molven

I wonder what kind of pipetting error, maybe not enough IPC captured? inaccurate pipetting? but why always the reaction from 1 - 5? I know its hard to tell without watching but what can I change or how can I improve my technique. Thank you for your help.

Hi, When I perform qPCR the internal positive control of the reactions from 1-5 show low or no signals, compared to the rest of the reactions (approx. 20 reactions are performed in one run). PCR runs were performed by colleagues several times with the same material and everything went well. I am not sure what is going wrong and I dont know why the first reactions are affected and the other ones are fine, I assume either pipetting error (not enough IPC) or inhibition of IPC. I am thankful for any advice.