I’m completely stumped on how to use the Kd to gauge relative concentration of bound vs free protein. I was told something along these lines (I used "conc." instead of brackets because they weren't showing up properly):
Kd = ( conc. A x conc. B ) / conc. AB
"If concentration A is fixed and relatively low, and concentration of B is lower than Kd, then there will be relatively low AB and mostly free A."
How does this make sense conceptually? I've tried to model it out, but I can't seem to get anywhere. Can anyone please help?
