Hello community,
Now, after a very long time, I have to enter a chemistry lab again. The point is to create a nutrient solution:
5mM KNO3 (potassium nitrate),
2 mM MgSO4 (magnesium sulfate),
2 mM Ca(NO3)2 (calcium nitrate),
70 μM HBO3 (boric acid),
14 μM MnCl2 (manganese(II) chloride),
1 μM ZnSO4 (zinc sulfate),
0.5 μM CuSO4 (copper sulfate),
10 μM NaCl (sodium chloride),
0.2 μM Na2MoO4 (sodium molybdate),
40 µM FeEDTA,
4.7 mM MES,
43mM sucrose
How to prepare the solutions individually, eg 1 liter with 5 mn KNO3 is not a problem. But I'm a little unsure how to "combine" everything.
Method 1: Calculate for all substances how much I need for the desired concentration in 1 liter, pack them all up in a container and fill up to one liter. There would probably be too little water here due to a certain displacement effect, but with such quantities, this is perhaps negligible.
Problem: The quantities are so small that we don't have the opportunity to weigh them, even with higher concentrations and more liquid.
Consideration 2: Put all substances in the desired concentration in 1 liter (eg with the help of dilution series) and then mix everything together 1:1, for example, 100ml of everything.
Problem: Here everything would be diluted again, right? Would I then have to create everything in 12x (12 substances) before mixing it?
I would be very happy if someone could help me, unfortunately, the last chemistry class was 8 years ago.
Thank you and best regards
