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felbamato

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    How to rescue a growth factor diluted in water

    felbamato posted a topic in Biochemistry and Molecular Biology
    Dear all, for a biological experiment I have acquired 10 mg of CHIR 99021 (laduviglusib). In order to obtain a 10 mM stock solution I had to dissolve the powder in 2.15 mL of Dimethyl sulfoxide (DMSO). Unfortunately, I tried to dissolve the powder in distilled water. That powder is said to be insoluble in water. In order to obtain again the powder I tried to centrifuge the solution (1500 rpm, 20 minutes), hoping that the powder would precipitate, but nothing happened. I would like to know how to dissolve that solution: I sm thinking to put another 2.15 mL of DMSO in the water solution in order to have a stock solution of 5 mM (which would be fine for me). What would you suggest? Thank you very much.

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