scuzfly

So how would I know how much EGTA is deprotonated if I have 0.2M EGTA in solution with 0.2M Calcium? I'm trying to find out how many EGTA I need to bind Calcium in seawater at a pH of 8.5 without losing the Calcium into seawater. I have immobilized the EGTA*Ca using alginate.

I'm trying to find the pKa for EGTA and google isn't helping much. Wiki says it's 6.91 Another article says it's 8.8 I'm using EGTA to trap calcium and its in seawater which has about 0.1g of Calcium already in it. I'm trying to maintain a pH between 8-10 without losing the EGTA bound Calcium. If someone could help me I'd greatly appreciate it. I'm trying to run the experiment asap.

I had an immunology teacher tell us all you'd need is to make something airborne, highly concentrated, contageous and stable so you could walk into an international airport with a small Binaca container and spray a couple areas. People would carry it onto planes without knowing it and spread it all over the world. Didn't think much of it until swine flu broke out and we saw it all over the USA. Then I started thinking of 12 monkeys.

Calcium Ions. I'm trying to get them to work like an enzyme and not get consumed. I'm doing a precipitation and it works with a lot of calcium added to solution however I want to do it using the calcium in the solution BEFORE I add it that way I can reuse the calcium I added again and again.

I have an interesting situation in which I need to find out how to immobilize calcium but retain its activity. Google just pulls up Sodium Algenate and Calcium Chloride is used in the last step to harden the beads. That's not going to work. I'm wondering if I could possibly charge calcium onto a sheet and have it stay there in water or if someone knows of a method I could try that would allow me to reuse the calcium.