hi all,
I've question
we have human gene (with its promoter , TE , ....ect ) which code for a certain protein inserted in plasmed of bacteria , but what happen is there was no expressing for this gene !!! ... why????
-I think that may be mRNA degrated before translation by the action of nuclease enzymes. in other words, no capping or cleavage make the mRNA exposed to 3' exonuclease & 5' exonuclease.
- OR may be because there is a gene control the splicing for this gene which is not transferred to plasmed. I mean there is other gene may code for protein control splicing in specific way for exons that stop the translation or produce inactive protein. is it possible ?!
I hope someone help me in this question .
Thank you ,
Jumana