Hi everyone, I'm new to the forum and have this question on biochemistry related to a homework assignment that I can't wrap my brain around.
So I'm told that a mixture containing the following two polypeptides were subjected to an isoelectric focus gel electrophoresis. Two bands were observed. I need to identify which band belonfs to each peptide, and briefly explain why.
The polypeptides:
Thr-Ala-Asn-Phe-Glu
Pro-Gly-Thr-Cys-Met
I have a diagram which shows a band at around pH of 5-7 and another at pH of 9-11. I know this procedure utilizes the isoelectric point of a protein, but so far in my study of biochemistry (1 month into the semester), I've encountered the isoelectric point of amino acids. I'm not sure about proteins. Basically, I want to understand how can I figure out the isoelectric point of a protein, thus be able to tell which band belongs to which polypeptide. I would super appreciate any help!
If you (reader) intend to respond, please do it fast because I need this before 2:40 PM
