Rusty123

Hello all, I am a current Biochemistry Graduate Student and need to pick a couple of topics to write research proposals on using the NIH research proposal format. I came on this forum because I have been searching for a couple of weeks now and I haven't found anything that interesting. I need some topics which that are current and could maybe provide up to 2 years of research. If you have any ideas or have seen any interesting journal articles I would appreciate it! Thank you. (Also, these topics would not be for my current research, we are supposed to write hypothetical research proposals so they can be on any topic.)

I would use the Henderson Hasselback Equation for the first 2 questions in your problem.

Can someone please help me answer this question? Thanks!!! 4.5 mL of a protein with concentration 48.2 microM is placed inside a dialysis membrane, which is then placed inside a beaker containing one liter of 20 mM HEPES buffer at pH 7.5, plus 14.5 microM of a fluorescent ligand. The dialysis membrane permits the ligand to pass through but not the protein. The solutions are allowed to set over night to reach eqilibrium. After equilibrium is reached, fluorescence measurements are used to establish that the total ligand concentration inside the membrane is 62.7 microM, and the total ligand concentration outisde the membrane is 13.5 microM. The protein concentration inside the dialysis membrane is re-measured, and found to be 48.8 microM. What is the Kd for ligand binding by the protein? I am not really sure where to start out, should I recalculate the concentrations and make them for the overall volume? I am confused .