Aisha_18

How would you detemine the length of an amino acid chain from a formalin titration of a hydrolyzed an unhydrolyzed peptide. My ratio of carboxylic groups came out to be 2.5, but how do i determine the chanin length givien this information?

Thanks,but i am not sure what volume to use given my the dilutions. Do i just use the 1ml for the ethanol? Or just use whatever volume of the ethanol substrate i would have used.

hi, I can really use some help. How would you convert ethanol substrate from 50 microlitres to micromoles/ unit? i dont know if this will help but the ethanol substrate was made as follows 1 mL of ethanol (95%v/v) was diluted with 199 mL of distilled water making the final concentration of the stock 100mM. 15 mL of this stock was thenplaced with 85mL of a buffer solution to make the final concentration 15mM. This stock was used for the enzyme assays.

hi, I can really use some help. How would you convert ethanol substrate from 50 microlitres to micromoles/ unit? i dont know if this will help but the ethanol substrate was made as follows 1 mL of ethanol (95%v/v) was diluted with 199 mL of distilled water making the final concentration of the stock 100mM. 15 mL of this stock was thenplaced with 85mL of a buffer solution to make the final concentration 15mM. This stock was used for the enzyme assays.