Sorry about that! I couldn't seem to get it in a decent looking format by copy-pasting, but here goes:
QUESTION: Mustard gas, Cl-CH2-CH2-S-CH2-CH2-Cl (also known as HS or Yperite) is an inexpensive, easily manufactured and highly effective blistering agent. Although fatalities are rare, victims frequently suffer blindness, agonizing burns, long term respiratory damage, permanent incapacitation, and drastically reduced lifespans. The agent is also persistent - casualties can result from individuals taking shelter in contaminated soil. You have discovered an enzyme from a rare species of Bolivian poison dart frog that inactivates mustard gas, converting it into an effective deodorant. The battlefield uses are obvious.
Your initial extract contains several proteins with the following properties:
Protein
Molecular Weight
pI
Shape
A
83,000
5.1
Globular
B
12,000
7.5
Globular
C
41,000
7.9
Globular
D
33,000
5.4
Globular
E
33,000
5.1
Rod-shaped
F
83,000
8.2
Globular
a. I apply the mixture to a chromatographic column containing an an anion exchanger (Diethylaminoethylcellulose) buffered at pH 6.5. Based on your knowledge of pH and pI, and of ion-exchange chromatography, which proteins will stick to the column at this pH? (5 pts)
ANSWER KEY: B and C have a negative charge at pH 6.5 and will stick to the column
ME: See, I would have said A,D,E because those proteins' pI's are below the buffer pH of 6.5, which would make them negatively charged, and therefore bound to the column. Right?
Merged post follows:
Consecutive posts mergedAnyone... Bueller...? This doesn't seem very difficult, I just think the teacher was wrong, which is throwing me off...
