sasu

can anybody tell me process for breaking the neutrophils:mad:

one simple que . we are using 5% dextrose & 0.9% NaCl solution as isotonic solution. but osmotic pressure of 5% dextrose 6.7 atm & 0.9% NaCl 3.7 atm. then why rbc get breaked if we use 3.7 atm of dextrose solution?

ok I am not intrested in observing the breaked rbc.... I want that all rbc should get breaked without causing much problem to wbc ....just now i am using ammonium chloride 0.8 M solution .Other method is chilled water. whether plane water:-p will cause any problem to wbc or not

if RBC is kept in hypotonic solution cell wall will break leading to death of cell. but what about cell membrane ? i know cell membrane is not able to maintain the inside pressure iam using ammonium chloride 0.8 m to break rbc .then also i am getting rbc under microscope ... how one can distinguish between intact:mad: & breaked rbc:eek:

i want to separate rbc & wbc by chromatography

i want to separate rbc & wbc by chromatography

can anybody tell me goood method for rbc lysis:embarass: