I have identified via bioinformatics a potential antigenic peptide in my protein that is possibly expressed on the surface of tumour cells. I am looking for "easy" ways (I am a non-immunologist) to see whether a synthetic peptide against this potential antigen does indeed invoke an immune response. I cannot use PBMC proliferation assays but have access to cancer patient seminal fluid which contain T cells (CD4/8 positive). Can i simply select these cells on CD3 plates, incubate with peptide and measure proliferation/IFN-gamma/TNF levels?
If not please suggest alternatives!
many thanks
natalie
