genefiend

by it i mean the gfp. you can insert it into the dna of bacteria with a heat shock therapy. you freeze it in liquid nitrogen, and then place it in warm water. theirs a 10 minute video on youtube. now can the gfp be extracted from the dna without restriction enzymes? i have read in some places that the some bacteria and yeast will excrete the protien into their growing media which makes it much easier to purify. the reason why i ask about the bacteria is because it wouldn't require refridgeration. Then again if i had to restrict it out of their dna it may not be easier... thanks for being patient with me, i know i dont know much, but i am learning quickly.

thats what i keep reading, but i dont know if i can afford a freezer that cold. what would be the difference between the coding and the actual gfp? also if i heat shock it into bacteria can it then be extracted safely?

Hello I plan on storing GFP in a -8 degree celsius fridge. how long before it degrades if The buffer used is TE? also i have heard the main problem with DNA going bad is the freeze thaw cycle from taking it out, using it, and resoring it. would you say its better to store in multiple 20 ul containors, or a larger contanor that has the frozen dna shaved off with a scraping tool like a sterile razor blade. genefiend.