Penny

If the yeast cells were cdc mutants before the UV irradation, then they won't be able to grow. Their cell division cycles would be disrupted. The cells would have to be wild-type before UV exposure. So................ would there be a "temperature" gene near the cdc gene? Upon UV exposure, both genes are mutated, causing the mutant cells to be also temperature-sensitive? LOL Pure guess. I hope I didn't go too far off the tangent. Thanks for your response, CharonY! Penny

Hi all, In one of my biochemistry courses, we went over the cdc (cell division cycle) mutant screen using conditional mutants (temperature sensitive). Yeast cells were exposed to ultraviolet light (to cause DNA mutations). Next, they were plated and incubated at 25 degrees, then replica plated and incubated at 37 degrees. The temperature sensitive clones were isolated and examined for cdc mutants. I am a little confused about the conditional mutants in general so my questions are... 1. What is the purpose of incorporating conditional mutants in an experiment? (I guess my question really is, how do they know the temperature sensitive clones contain cdc mutants?) 2. How exactly is it done? (If the explanation is really long and you don't want to do it, you can point me in the direction of some papers =D.) Thank you so much! Penny

You need to basically indicate whether the groups are pointing out or into the page. Therefore, you need to use R (clockwise) or S (counterclockwise) to label the chiral centers.

Live off this? I, on the other hand, would rather die.

I should rephrase my question. Why chew anything in the first place?

They are the same. It's just a different way to represent a methyl (-CH3) group.

Out of curiosity, why would you want to chew tea as a tobacco substitute anyway?

I think there is a published chart to predict a person's vital capacity when given age and height. However, without knowing the inspiratory and expiratory reserve volume, the vital capacity won't be very useful. Determining volumes (i.e. tidal volume and stroke volume) from rates seems a little opaque to me. I'm not sure if this could be done either...

Read the original post carefully. The diagram isn't what it seems...