geniusgene

Thanks for your reply. Yes we target 16S rRNA sequence for E.coli genomic DNA whereas antibiotic resistance gene in plamid. But that required two set of reaction in quantitative PCR. I was looking for a universal sequence that would help me to perform the test in just one reaction.

No.. I just want to detect E.coli DNA contamination in my sample

Is there any strech of sequence that is common in E.coli genomic as well as plasmid DNA? I am trying to find a target for PCR using universal primer against such a sequence so that it will help me in detecting the presence of genomic as well as plasmid DNA in my sample.