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pedore

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Everything posted by pedore

  1. I'm not positive about this, but I think it also has something to do with the higher rate of asexual reproduction in plants. Accidental polyploidy in an animal species is likely to render that individual infertile, and unless the animal is capable of reproducing through parthenogenesis, they will not be able to pass the polyploid condition on to any offspring. Plants are more likely to be able to create 'clones' through budding, fragmentation, or other types of asexual reproduction, and so more polyploid individuals may be created. This might allow the plant in question to undergo other processes that could make it fertile again (chromosomal rearrangements of some sort). Of course, this doesn't begin to cover the fact that Cypress brought up - that polyploid animals are formed but usually do not survive to maturity. So obviously there is more to it than that, but I thought I'd throw it out there.
  2. Hi everyone, I'm hoping someone here has used HRM to do SNPs. After reading some of the literature, I am a little confused about a few things, and if anyone can answer any of these I would very much appreciate it! - can HRM only be used for one SNP at a time? Is there any way to multiplex? - do you need special primers, or can you use any of the ordinary primers you can order online? - is this really better than real time PCR to do SNPs? Speaking of real time PCR, can you do more than one SNP at a time that way either? Thanks for any insight anyone has!
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