Does anyone know the sensitivity range of nested pcr ?
I tried goggling but could not get the answer .
I am from INDIA .
Is nested PCR sensitive enough to detect the acute infection Hepatitis c infection between 4-6 weeks ?
I did the two pcr and got condractory relsults.
This answer will help me a lot because , I had HCV PCR done realtime PCR got results as "HCV RNA DETECTED" on fourth week after exposure . And the lab which i did the first was not a very famous one.
As many said that my risk factor was very very unlikely to cause infection . After a week of first pcr , I did NESTED PCR RNA in the most reputed lab In my state which has got College of American Pathologists (CAP) Laboratory Accreditation. And got Results as "HCV RNA NOT DETECTED".
I belive the testing done in second lab and there would not be any errors in testing method there .
But i need to know , IS nested pcr sensitive enough to detect acute infection at 5 weeks post exposure ?
If anyone know the sensitivity range of nested pcr ( or ) the sensitivity diffrence between the realtime and nested pcr methods say me .
The answer would reduce my anexities and stress .
