The aminoacid glycine is often used as the main ingredient of a buffer in biochemical experiments. The amino group of glycine, which has a pKa of 9.6, can exist either in the protonated form ( -NH3+) or as the free base (-NH2), because of the reversible equilibrium
R-NH3+ Û R-NH2 + H+
a) In what pH range can glycine be used as an effective buffer due to its amino group
B) In a 0.1 M solution of glycine at pH 9.0, what percentage of glycine has it’s amino group in the NH3+ form?
c) How much of 5 M NaOH must be added to 1 L of 0.1 M glycine at pH 9.0 to bring its pH to exactly 10.0?
d) When 99% of the glycine is in its –NH3+ form, what is the numerical relation between the pH of the solution and the pKa of the amino group? (for example, “the pH is double of the pKa” or “the pH is 1 pH unit above the pKa”, or “pH = pKa + 1”, etc.)
I dont know where to begin.