I am currently busy with enzyme assays (well, trying to be): This is what I am doing, but its not working...
I induce mouse hepatoma cells with ethanol and a test compound. The test compound is supposed to cause the transcription of my enzyme which I am supposed to measure the levels of after cell lysis, freezing and thawing. However, after measuring the OD, there seems to be NO significant difference between ethanol and test compound induction, i.e. I generated two identical looking curves. This is so frustrating.