chantel.bok
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Does anyone work with or understand PAM fluorometry for the purposes of assessing ETR rate in microalgae? We have a Phyto-PAM, but not sure how to operate - everything is set up, but how to use the software to get the correct results is a bit shaky. I think if I understood what results I am supposed to be getting, then I will be able to play around with the settings - but I am completely new to this. And no one else at work seems to be able to help me either... I've attached the manual of the PAM we use. Thank you! phyto_4e.pdf
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Optimising an Enzyme Assay
chantel.bok replied to Monique's topic in Biochemistry and Molecular Biology
Have the results been 'reproduced' before - more than one reference? If you are doing the same thing as was done before (exactly), you should be getting the same results. Otherwise, there has to be something wrong with the compound. You are using the same concentrations and everything? -
Optimising an Enzyme Assay
chantel.bok replied to Monique's topic in Biochemistry and Molecular Biology
It's likely that there is something wrong with your test compound, otherwise is it absolutely certain that this particular compound causes expression of the enzyme? -
Indeed we shall do so. We can discuss it over a glass of red wine, good for the heart.
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It was good to have seen you today Monique! I particularly enjoyed our in depth discussion on certain genetic disorders that seem to have become more prevalent. Or perhaps it is our awareness that have become increasingly sensitive.