what are the protocols for the measurement of enzyme assays of inflammation (GSH, MPO, MDA, SOD and catalase) with a spectophotometer without using kits?
please i would like if anyone can send me protocols on how to carry out MPO, SOD, GSH MDA assay with the use of a spectrophotometer and a microplate reader. also protocols on how to assay for catalase. i dont have the assay kits, i would love to get a detailed protocols on how to get or prepare the necessary buffers for the aforementioned assays. thanks
i need assistance on what models or protocols to use for enzyme assay such as catalase (using a microplate reader), glutathione, superoxide dismutase, myeloperoxidase and lipid peroxidase. i want t know how to do these assays without the use of enzyme kits but use of buffers and how i would prepare those buffers and also how to prepare my samples for these assays.
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