KAZU

Is there an assay for yeast to see if they are haploid or diploid?

I don't know if this helps, but people in the micro brewing industry use a method called Flocculation. http://www.crc.dk/flab/newpage4.htm is a good link that explains the process. This process has also been used for e coli http://www.springerlink.com/content/r81802m228k574q6/ Kazu

Huh, I thought electron welding and plasma cutting where essentially the same, I mig weld almost everyday and to get better penetration on thick material, I usually prep the material by preheating it with a torch, or cutting into it so I can dump more weld in. In electron welding, I thought the advantage was not having to do the prep to get penetration or no foreplay as my machinist friend says, since it acts like a plasma cutter in the way of "blasting" apart atoms and shooting electrons into the material resulting in excitation heat/fusing of the material. As far as shielding the weld goes wouldn't it be cheaper to shield with an inert gas like mig or tig welding? Do you know what the benifit is by sheilding with plasma? The only benifit I see is that it could maybe heat prep the material first, but again that can be done with a torch.

I run a plasma cutter thursdays and fridays, the thing cuts through 1/2 inch plate steel like nothing. I can't wait til we all have lightsabers.

Did you trouble shoot your transfection? What kind of transfection, superfect? lipofectamine? Ca? With pcDNA 3.1 I know forskolin is a good control of transfection efficiancy. What kind of mam cells? Are you testing the mammalian cells? Or is this to mass produce the protien for development of an AB? If you are not worried about glycosylations, go with bacteria.

Try a different water supply. We had a contamination issue a while back and we sterilized everything, but contamination was do to the corrupted Milli Q system. Take your water sample streak it, pH it, whatever.

I took a survival course and appearantly the cure for the sting is in the root. Pull the root out and apply to effected area and pain goes away. I've tried it and it seems to work, but it could be purely mental.

My organism is not fully sequenced, there are partial sequences available in the trace archives. I will also look into the hybridization techniques, I just don't want to go there, I'm way too impatient.

Sorry, to clearify my personal definition of building a contig. In this case I define it as exctracting partial sequence from a data base and then blasting it against the genome of a specific organism. Then analyze the output sequence alignment by blasting sequences that partially align with the original sequence against the entire data base to check for homology then manually aligning the sequence overlaps to create a more complete sequence.

Charon, the problem with that is there is no way to know if it is specific for the gene I am interested in since the gene I'm interested in might not exsist in any database......yet. If it does exisist it would only be in a trace archive packed full of introns since not all animals have its genome sequenced. So I would need a good chunk of sequence.

I'm new here and I have no idea if this is the correct forum to post this thread on or not, but here it is. The first question: How do I clone a novel gene that encodes a protein that has sufficient evidence to support its existence? Since I don’t want to screen libraries, my first thought is this: 1) Compile sequence alignment blocks of known homologous proteins. 2) BLAST against whole genome of chosen animal or trace archive. 3) Build a contig and use it to design GSPs. 4) Test the GSPs with genomic template for animal of choice. 5) Go into 5’ and 3’ RACE. The second question: If I decide to go with my first thought, how do I do parts 1 and 2? I suck at computers.

What up. I'm new up in this piece. I like long walks on the beach, watching sunsets and molecular neuroendocrinology.