What is the essential difference between two strains of E.coli: BL21(DE3) and TOP10? Why is BL21 used for protein expression and TOP10 for plasmid storage? The plasmid in this case is pDEST17. I'm really new at all this and looking at genome of these two doesn't give me any answers... I know it has something to to with lack of lac operon in TOP10 strain. Please help.
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