Cesco_NZ

Thanks for your answer do you think that using streptavidin conjugated with a resin or beads should be ok? then i can elute... and how i can quantify the Biotin-SiRNA conjugate? Francesco

Hello everyone I am going to attach an SiRNA to a functionalized biotin. Then i need a way to quantify the efficiency of the reaction and i tough that a fluorescet Streptavidin could be usefull The problem is that i don't know exactly how to run the essay I tought that i can attach the SiRNA/Biotin somehwere and then reveal it by streptavidin Any suggestion? thanks a lot Francesco