hypervalent_iodine

! Moderator Note For the benefit of people posting here, overtone has just been suspended for 31 days and will not be participating in discussion until this time is over.

overtone has been suspended for 31 days following his continued refusal to pay attention to countless moderator warnings about soap boxing and backing up claims with relevant citations.

! Moderator Note copernicus, Since you are clearly not interested in discussion, this thread is closed.

Hi. I'll start by introducing myself. I am hypervalent_iodine and I'm a moderator here at SFN. I am also 25 years old. Let's begin by realising that a 'theory' is not proven correct just because someone who cannot show any evidence for it thinks it makes sense. Math probably is needed to determine if it is correct. In any case, you're only allowed one thread per topic so this is about to be closed. Deal with it. That's how science / this forum works.

! Moderator Note Kramer, Your posts were off topic and your continued efforts to drive conversation towards discussion of your own ideas are considered derailment under the forum rules. I have split all of your posts and replies thereto into the trash. DO NOT attempt to derail this thread further by discussing this moderator note.

The forum isn't for advertising, sorry.

Thank you for the suggestion! I'll check if the library has them. I was working with RNA transcript libraries from various non-model species for sequencing on the IonTorrent platforms. Maybe not as difficult as the older emulsion PCR technologies, but boy did they not want to play ball 99% of the time. I think the lab has a Nextseq now, which does seem trivially easy in comparison.

Nope. If you want help on your homework, post a thread with your specific questions in the Homework Help section and someone will help you, but no one here is doing your assignment for you.

The use of disposable diapers is obviously a pretty recent thing, but I would imagine the use of cloth is not so old either given how expensive it would have been. I found a few randome websites with some timelines: http://www.hellobee.com/2012/07/20/a-history-of-diapers/ http://www.diaperjungle.com/history-of-diapers.html The main idea being that prior to cloth, which seems to have come into popularity amongst the masses last century, plant matter and furs were the done thing. I also came across this, which I thought was pretty interesting (or as interesting as diapers can be). A few comments I read mentioned that this was a pretty common practice in poorer areas of places like China in lieu of available diapers.

I have a somewhat rookie request. Recently I was offered a 2 year research position in a lab funded by the Gates foundation. My work will primarily be with a certain species of algae and working on ways to optimise output of various commercially valuable fatty acids, though there will also be some organic chem work in there somewhere for the pharmaceutical branch of the institute. Most people who have been here long enough will know that my background is in organic chemistry. I have a decent enough understanding of the broader concepts of molecular biology and have some practical experience in genetics labs, though it was limited to RNA extraction, library preps for NGS platforms and cleaning up and running samples for Sanger sequencing. I don't really want to go into this new position unprepared or unable to contribute to my own work intellectually (though I'm sure I will pick it up as I go) and so I was hoping someone would have a good recommendation for a practical molecular biology text. I'm looking for something similar to what the Vogel text is for chemistry or like another text I've read, From Genes to Genomes, which I found invaluable for troubleshooting and modifying my experiments while doing undergraduate research.

This isn't a reason why a crank like Dr Oz wouldn't put it on his show or why people aren't raving about it, but diets are in general a bad and sometimes misleading and stupidly expensive way to lose weight. They are short term since more often than not, the weight loss achieved on a diet is put back on when a person finishes the diet and goes back to their regular habits and may also be damaging. There really is no better substitute to a balanced, healthy lifestyle of good food and exercise. One specific point about your post, though: how does one, 'gleen water,' from burning fat? As far as I recall, water is not produced in this process in the body but is used up instead in one of the reactions.

! Moderator Note Moved to Speculations.

! Moderator Note Seriously? Quit the spamming, stick to your already existing thread on this topic or we will ban you.

! Moderator Note STOP OPENING MULTIPLE THREADS ON THE SAME TOPIC!

Please confine conversation on a single topic to a single thread. Since this is identical to a post made in a long running and existing thread, this thread is closed.

As John Cuthber has said, you really can't justify the structure from just the molecular formula. Molecular formula can represent many different chemical structures and you have no way of knowing the correct one for your question without much more info. Is there any reason you are trying to do this?

You can't get much structural information beyond what's in the compound and double bond equivalents from a molecular formula.

! Moderator Note One thread per topic. And please keep the speculations in the Speculayions section.

I think you're confusing electrons with ions. An ion is an element or polyatomic species that is charged - I.e. Has a net loss or gain of electrons. Mg has two valence electrons and will typically lose both of those to give Mg2+. If you can work out the charge (the 2+ in the case of magnesium), working out the combination of that plus a halogen to give a neutral species (a compound where the combined charge equals 0) is relatively easy to do. Take Mg2+ and Cl- for example. To create a neutral species, you need two Cl- ions for every Mg2+ ion, giving MgCl2. This is a very simplistic way of doing it, but since you seem to be dealing with simple compounds it should be fine. As for working out the charges, there are some general rules for certain groups of elements and the rest you can work out from there. - Elemental forms will always have an oxidation state / charge of 0. This is for things like O2, H2, Na (not Na+), etc. - hydrogen, unless in elemental form, has a charge of +1, as do the group one elements. - group two elements have a charge of +2, unless they are in elemental form. - oxygen is always -2 unless it is in a peroxide, in which case it is -1, or elemental form. - halogens are always -1 unless in elemental form.

What do you mean by the value?

How familiar are you with determining charges / oxidation states of ions? As in, if I asked you what the charge on Na is in NaCl, would you know it or how to figure it out?

You need to review this. I asked if it would grow in the +Hec media. Yes and no. It means exactly what it says, really. I think you are struggling with what a loss of function means in this context and helping you beyond what has already been said to you would mean giving you the answer, which is anti to the purpose of this part of the forum. Please have another read through the thread and what a loss of function means.

I don't really see where math is involved in this question? Label the colonies as a, b, c, d, etc., if you like, it makes no difference. Think about it some more. The plates labelled +Hec contain media supplemented with whatever the Hec nutrient is. So if you have something with a loss of function in the Hec gene, would you expect it to grow there or not? What about the colonies with the other loss of function - would they grow in that media?

Look, it's not our job to do your assignment for you. I can only assume you've had longer than the last few hours to do this and if not, then certainly longer than that to review the content before attempting this. That you have so little time left is not our fault and I have no sympathy for your predicament. No, you do not add numbers. Do some research on what a loss of function mutation means if you are still struggling with the concept and have another read of chadn737's post.

A loss of function mutation is a mutation that causes whatever gene the mutation is in to have attenuated functionality (or none at all). This would be something done prior to growing them on the media in the question. So, your answers are incorrect. If 1, 2, 4 and 9 had a Hec loss of function, then why would they not grow on media supplemented with +Hec?