mariacervantes888333

In ion exchange chromatography, how do you find the order of elution of proteins based on pI? I have a graph of KCl conc vs. fraction number and I have to find the conc of KCl required to elute some given proteins. And I'm given pI values of 4.6-4.9 for albumin and 6.8-7.8 for hemoglobin, and 10 -11 for cytochrome c, so what do I do with these numbers in relations to my KCl conc std curve and my ionic conductivity of fractions?