Fill in the blanks.
The first workable fraction in the enzyme purification is the __________. Each subsequent successful purification step should show an increase in ____________ and very little decrease in __________________. To keep track of all proteins during each step, we would typically run a general protein assay, such as the ________, which utilizes the dye Coomassie Blue, which binds to ___________ groups in proteins.
What I have so far:
1. Crude
2. Not sure if it's specific activity or fold purification.
3.
4. Bradford assay
5. Hydrophobic