ho&ho

hi, anyone could shed some light on this puzzle pls, how to estimate when in evolution did a ncestral protein arise, in other words, How old is the ancestral protein likely to be? detail as bellow, there is a amino-acid sequence of part of the AntP gene product , KRGRQTYTRYQTLELEKEFHFNRYLTRRRRIEIAHAL Use this to do a BLAST search to find other proteins related to Drosophila AntP. First open the BLAST page at NCBI. Choose the Standard Protein Blast option (BlastP), and copy and paste the sequence into the search box. Click "Blast!" to do the search, then click the Format button to see the results. This will give a long list of proteins that match the sequence, either approximately or exactly. How old is the ancestral protein likely to be. i v got the long list of protiens and the distance tree, however , i can tell how old it is . thanks

got it , that is why to compare with genome sequence... thx u very much indeed.

thx a lot. i m much clear at #4,5,6 by ur post ,but still wondering how to sort #3 via comparing to genome sequence? i think the #3 is about the location where the mRNA is produced, ist it?

anyone can shed some light on these puzzles as bellow, thanks. Microarrayanalysis has identified 3 previously uncharacterised genes that are strongly up-regulated in embryonic stem cells exposed to retinoic acid (embryonic morphogen). what assay can be applied to identify : 1. How many splice forms of each gene? 2. How big are the splice forms? 3. Where do each of the mRNA’s produced start and finish 4. Are they expressed in the developing embryo? 5. Where are they expressed? 6. Are they expressed in adult tissues and if so where? i think 1. northern blotting/ AgaroseGel Electrophoresis / 2. 3. In situ hybridisation, but which one would be better, Sectional analysis or Whole mount analysis? 4. 5. 6.

2.specific activity or purifiction (i think both r right) 3. yield