cc4280

Hi, I'm currently using Q-PCR to look at changes in gene expression under different experimental conditions. However, for one of the primers I'm using, I get a very noisy baseline. As expected, the noise increases as the primer concentration is reduced, but I don't understand why the noise is so high when the Ct value is still quite low. Are there any factors that contribute to a nosiy baseline? contaminations in the Q-PCR mixture perhaps? Any help would be greatly appreciated.