I've been trying to measure IL-10 production in CD4+ lymphocytes without any luck. Unlike other cytokines this one proves very difficult. Does anyone know how it can be done? The reason i can't use an ELISA with serum is because i won't be able to distinguish which cell (monocytes or lymphocytes) produces the IL-10. The other problem is that it has to be a quick test (no more than 24 hours or a max of 2 days). I know it has already been done by using CD3 and CD28 stimulation for a week followed by PMA stimulation of 5 hours. However that is something i can't do. So anyone any suggestions?
