sonicscorch

Here's my outline method: • Fill 14 clean test tubes with 10ml of 10% hydrogen peroxide solution, using a small pipette and 100ml measuring cylinder. • Leave one of the test tubes at neutral pH7, and check this using a digital pH meter. If the pH does not initially register at 7, then buffer solution should be added until the solution is completely neutral. • Then, monitoring the pH with the pH meter, add either sulphuric acid or sodium hydroxide to each of the remaining test tubes, so that there is a hydrogen peroxide solution that is each pH ranging from pH1 to pH14. • Add 5ml of catalase to the first test tube, and immediately attach a bung and gas syringe to the top of the test tube. • Use a stopwatch to time the reaction for two minutes, before recording the amount of gas that has been produced and removing the gas syringe. • This should then be repeated for the remaining 13 test tubes, until results for all 14 pHs have been recorded. Cheers. Any information would be helpful.

Hi guys. I'm an A Level Biology student in the UK, and I've just done an experiment to find the effect of pH on catalase. I used powdered catalase and expected the optimum pH to be 7, which I could have explained... But it came out quite clearly as 10, and I have no idea why, as it contradicts the research I have done on catalase. Can anyone explain this to me? Because I have to write it up and right now i'm completely stumped. Cheers.