Here's my outline method:
• Fill 14 clean test tubes with 10ml of 10% hydrogen peroxide solution, using a small pipette and 100ml measuring cylinder.
• Leave one of the test tubes at neutral pH7, and check this using a digital pH meter. If the pH does not initially register at 7, then buffer solution should be added until the solution is completely neutral.
• Then, monitoring the pH with the pH meter, add either sulphuric acid or sodium hydroxide to each of the remaining test tubes, so that there is a hydrogen peroxide solution that is each pH ranging from pH1 to pH14.
• Add 5ml of catalase to the first test tube, and immediately attach a bung and gas syringe to the top of the test tube.
• Use a stopwatch to time the reaction for two minutes, before recording the amount of gas that has been produced and removing the gas syringe.
• This should then be repeated for the remaining 13 test tubes, until results for all 14 pHs have been recorded.
Cheers. Any information would be helpful.
