Cyn

Hi.I'm trying to stain murine eosinophils from cytospin preparations. I'm using Dff-Quik that is a modified version of Wright technique. I've got many problems to obtain a clear preparation of eosinophls, with their red cytoplasmic granules and their multilobulled nucleus because I don't know if I'm washing in excess and my eosinophils get unstained. Please if someone has experience I will aprecciate any help. Thanks