I want to genotype E18 mice as fast as possible. Preferably within a couple of hours.
I suppose the bottleneck of this all will be the tissue digestion/ DNA extraction.
Does anyone have experience with this and/ or can provide with some digestion tips? What tissue you use? what buffer you use?
Fastest I did it until now was 2 hrs , shaking @55 celcius with:
10% Triton
10x MGB
H2O
Proteinase K
Thans in advance guys!
We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.