ggallin

If I have a enzyme 50kDa and I know that the concentration of said enzyme is 140pmol/mg in a total protein conc of 12.785 mg/ml how do I convert the amount of enzyme to grams? Further, if I add 100ul of the total protein to a total volume of 2000ul, how much enzyme will I have in the containter???? I am unterly confused when it comes to unit conversion!!!

I am just not understanding how these things work. Using this plot as an example (attached). Can someone explain how one would go about generating this. I mean obviously you dont have the raw data to generate it, but in theory? Thanks.