Amela

Ok, Thank you, Then any tips to get rid of the RBC that persist in the PBMC solution after Blood separation with Ficoll gradient???

The Permeabilisation solution that allows the antibodies to enter the cells could affect the integrity of the membrane and thus RBC may die cos of that, I was just wondering if the fixation solution could prevent this?? Some of my colleagues in the lab noticed that after ICS experiment RBC are reduced.....

Hello there, I wonder if the FIX/PERM solution used during the Intra Cellular staining Could remove or kill red blood cells that could be present in the sample??? Thank you

Hello everyone, I was trying to stain T cells for memory markers(CD45RA,CCR7) and then I got for some samples 3 populations of CD3+ T cells , Those populations are different in their CD4/CD8 ratio and also in the % of memory subsets Any explanations for those 3 different populations???? Thank you