HuskerNation
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Inducing UV mutations in a gene
HuskerNation replied to Ivaylo's topic in Biochemistry and Molecular Biology
Just another thought on this, considering my master's thesis is on UV mutagensis in marine bacteria... You will want to use germicidal (UV-C) which is in the range of 190-290nm. This is the type of damage which causes direct damage (pyrimidine dimers, 6-4 photoproducts, rare base adducts, etc.). If you use UV-A (320-400nm), you will only get indirect damage via reactive oxygen species, and not even necessarily in the DNA, but also could effect enzymes and other proteins.