ashokprabhus

1.how to distinguish/separate between two light chains of immunoglobulins as they are similar in all respects-ie., they move as asingle band in SDS-PAGE how do we know on the 1st hand that 2subunits of light chain are there in the same band 2.why and how does a A+ve person have Anti-B-antibody in his sera even without prior exposure to B antigen 3.why dont we use urea in place of sds in doing PAGE?