a_hublikar

The cells undergo Lysis,and sometimes the cell debris is seen under the microscope-(phase contrast). Thanks for your reply,I will give a try as u suggested.

Hi all, I am engaged in Industrial fermentation of many bacilli.Recently I am having some problems with B subtilis.During fermentation the bacterial cells start multiplying and immediately get disappeared. I am using the same culture since long,earlier I used to get very good batch and now I am not facing problems. Can anybody help me in this regard?