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Thanks, Dr. DNA. Your response makes a nice sense. Sorry, CharonY. It was me who misunderstood something. people in the hospital clarified this for me as well. Thanks again, CharonY.

Dear Dr. DNA, Is it possible that UVC-reisitant bacteria do not resist to antibiotics? If it is case, the UVC mutants may not survive in the selective media with antibiotics anf the mutants may not be selected? Please advise. Thanks a lot. Dear CharonY, I was just told by our hospital (MGH, Boston, MA) that the DNA sequencing only costs $0.40 per run here. We have this service in our hospital. http://www.hpcgg.org/Sequence/submission.jsp?name=servicesforresearchers&subname=sequence

Dr. DNA, Thanks a lot. It's very helpful. Dear CharonY, Thank you very much for your suggestions. Actually I am designing this experiment (this one of the experiments) for a grant application.

Dear Ecoli, I really appreciate your comments and suggestions.

Dear Dr. DNA, Do you mean the mutations will make the bacteria more sensitive to UVC irradiation? If it is this case, what might be the reason for indcuing the resistance? Please advise. Thanks so much!

Thanks, Paralith! Thanks, Ecoli!

Dear Paralith, Thank you very much for kind response. It's really helpful. Could you recommend to me any written materials (e.g. publications) on how to perform the sequencing of the whole genome of the bacteria? Thanks again!

Dear everyone, I have basic a question about detecting DNA mutations in bacteria. I am investgating whether bacteria (P. aeruginosa) can develop resistance to UVC irradiation . If resistance develops, my hypothesis is that DNA mutation is induced by UVC irradiation. How to prove this? Sorry for this basic question, becuase I am an bioengineer and DNA questions are out of my field. Thank so much!