Hello,
I have a question regarding regulatory experiments using generated regulatory T cells and adding them to different DC:T (responder) cells allogeneic cultures. The problem is that by adding Tregs, you also dilute the existing DC:T(resp.)cell ratio and thereby automatically reducing cpm counts.
Does anyone have an idea how to cope with this, becasue in the literature they don't seem to have a problem with it, but I do. When they put control effector cells instead of Tregs, it doesnt lower their cpm count, although the number of effector T cells is as high or higher than that of responder T cells.
Thank you very much,
best regards.
