I'm doing an experiment to measure the light absorbance of bromophenol blue? (spectrophotometry)
i have 5 test tubes: 1st) 1ml BB and 4ml H2O 2nd) 2ml BB and 3ml H2O 3rd) 3ml BB and 2ml H2O 4th) 4ml BB and 1ml h2O and the 5th) 5ml BB and 0ml H2O.
max absorbency at wavelength 595 nm
concentration of stock solution: 10mg/L
results are: 1st tube: .332
2nd tube: .368
3rd tube: .375
4th tube: .533
5th tube: .746
how can i draw a graph concentration of bromophenol blue (x-axis) and absorbance (y-axis)? then i need to find the absorbance at concentration 2, 4, 6, 8, 10?
please i need detailed steps