tina87

Hi! I'm new to this forum and would greatly appreciate any help or advice on this topic!!! I'm currently in a microbiology lab course in college and we have to identify an unknown bacteria. I have performed a series of tests on my unknown and I really believe it is Salmonella. However, there is still some doubt because some of my results don't match typical Salmonella reactions. So I was wondering if someone could confirm my theory or if they could lead me to a helpful website because I have searched for hours trying to find everything! I just want to be 100% sure that I'm correctly identifying this bacteria. Here are my test results: Unknown Bacteria: bacillus with "rotten" smell Gram Stain: (-) Catalase Positive Amylase Negative Caseinase Negative TSI agar slant: color of the butt changed to yellow there was a black cluster at the bottom there was also a bubble on the side of the tube SCA agar slant: Citrate Negative MacConkey Plate: agar changed to a brownish color colonies were white/colorless MSA Plate: no reaction LevineEMB Plate: colonies were dark velvet with a metallic sheen agar was still dark maroon color Thioglycollate media: growth at top of tube (lots of turbidity) pink tint disappeared My main concern lies with the reaction on the LevineEMB Plate. The only bacteria I found that had that kind of reaction was E.coli. However, E.coli doesn't generate H2S (black cluster) in the TSI agar slant - so I'm very confused! Could there possibly be another bacteria that I haven't found? Any advice and/or help will be greatly appreciated!!!! Thank You!!!!