Hi to all,
I am a student from Germany, working in the lab of Neuroimmunology.
I am checking the expression of a cluster of differentiation (CD) protein in different types of genotype mice. For the same, I am using western blot method.
I am facing a problem in my western blot experiments is that I repeated my experiment several times but every time I get different tendency of protein expression among the different genotypes. For the control purpose I use alpha tubulin as an endogenous control, but the solution have been persisting any ways. Thats the reason I can't make the conclusion out of it.
I would be happy if I get the solution for my problem from you
Thanks
Enjoy!
