Sly3000

Thanks for your reply. Well, if you add too much to the culture the cells can also die. So that's what I want avvoid. I've added 1 microg/ml and that's a whole lot more than you suggest!!!

Hello all. I was wondering how much (what concentration) of LPS per ml of cell-medium, do you have to use to keep the PBMC culture alive and proliferating. The PBMC is from a healthy donor. Thanks in advance.