jorge1907

Feeding studies of salmonella are a matter of older published record and more recent retrospective epidemological data indicate 7 cfu (ID 50 - infection) to 36 cfu (ID 50 - illness) as effective levels. Lab strains are usually less pathogenic. I'm not aware of data relevant to cocnentrated levels.

Get a copy of Standard Methods for Examination of Water and Wastewater. Curious - why do you think the container will make a differecne?

Can you give us a reference and conext for this "salmonella fluid"?

The appropriate consideration would be growth on surface of appropriate agar medium.

MSA is applied typically to differentiate among the staph - with S. aureus producing acid from mannitol in presence of high salt. I agree with charon inn that color not withstanding, the streak does not appear to be S. aureus in colony morphology or and degree of color change. Have you performed Gram stain and catalase ? Coagulase?

i i'll narrow my comment - SMF -please provide th stoichiometry that defends the application of these technologies to reverse or even stop global warming.

Jesse's right. Penicillium roqueforti isn't know to product penicillin whereas P. chrysogenum and P. notatum do.

If you want surface area - use plates.

SMF - you infer that Gores book was a scientific text. It was and is not nd I'd like to see the proof that his work was vetted among climate scientists beyond a few who shared his immediate level of concern or those of Univ of East Anglia. i will note that one of the his fellow Nobel recipients noted that he did not agree with Gore's dire predictions, noting that he (the scientist) had often been wrong in predicting conditions that far in the future and thought it was unwise for Gore to do so. Global warming/climate change - to the extent that it is and will continue to occur leave us as little more than observers. There has not been a reasonable stoichiometrically-relevant solution proposed short of, as Lee Thomas of EPA said once of remedying LA pollution in compliance with the 1st Clean Air Act provisions, "tearing the social fabric." In a global warming remedial sense, Prius', green buildings, new light bulbs, wind mills, etc, etc are little more than environmental fetishes. And please don't think me unimpressed that you are a science teacher!! wow!

Poor pippo!!! Pity - he wasted his time (again) on HIS subject that has so little merit that even the pippo him or herself admits it's nothing of substance. But pipo's time is likely worthless so its waste is not a concern but a very entertaining rant. My apologies - tens of thousands of gallons of exfoliant cosmetics - not the HDPE exfollant alleged to be a problem. So a small percentage of even that amount - so small of a global scale as to be of vanishing importance - even to pippo. Let me help you - I am in the industry and known that not all exfolliants even use the HDPE about which the aussie activist was whining. Some use microcrystalline waxes. The issue is that resources are wasted on a subject trivial on its face so a publicity-seeking activist can get his name in the press. Invest resources in real issues - not silliness that serve no better purpose than to allow a childish rant of the pippo on a so-called science forum. Got it? Now go watch TV little guy - you're a great help to the universe.

I'd say it's pretty sophomoric - presuming simple transtions and "huge" quantities are possible. Huge quantities of rumen microrogansimss (and they're not just bacteria) - killed - somehow their DNA got into human intestinal bacteria and transformed to establish that capability - multiple time. Apparently the end product is some highly modified form of human intenstinal flora that would be maintained and digest cellulose all the way to simple sugar - with no detrimental effect on other normal flora or the host.

Exfolliant plastic (assume HDPE) is so environmentally trivial a matter as to be a total waste of time as we address issues. They are not made as fluid - the "gallons" are suspensions - usually aqueous. I'm not aware of data saying they go into oceans. As particulates down the drain waste going into municipal water treatments systems, they should end up in sludge. Even if discharged 100% into surface waters that eventually arrive at marine environments, the dilution factor willl be profound. Browne's alleged work (I've been unable to find anything but his news releases and interview comments) apparently exposed marine invertebrates to particulates and noted uptake - a bit fuzzy on effect. Even looking at these brands (and other exfolliant products) on your store shelves would reveal the tiny relative amount. What are the data that says exfolliant material exists in the environment at an environmentally significantly level?

The numbers have changed in your messages marc. Indeed if you went from ~10E5 to < 10 - you have a much more substantial reduction than within the range in your prev message (190,000 from 450,000). Assume multiple reps. Tell us about your treatment and control.

the property marq porposes fpr bamboo kun is that it's a bacteriostat (there is no word "bacteriostaticum") and it is the property to inhibiting growth of bacteria. A bactericide kills bacteria and that is what you are apparently investigating. as charon informed - (survivor #/original #) x 100 = % surviving. but charon was completely wrong in the computation "10/190000*100 is completely wrong by the estimated counts you offered 190,000/560000 X 100 = about 33% survival or ~ 67% kill. Importantly this level of kill falls into the standard error one typically sees in such assays and says the stuff really doens't work well to kill bacteria - if at all. charon - please be careful not to mislead

The lead in toys was a political issue, raised against the sitting admin in an election period. It had nothing to do with health - no one was injured and the exposure was of no consequence. The milk (presume melamine) did lead to injury and I think death. The question would be knowledge and motivation. A fine is hardly appropriate any more than should be the consequences of the violations for salmonella in peanuts.

What uninformed clow3ns - you can't get DNA from fossils you idiots!

Sperm stain with the Gram stain http://content.karger.com/produktedb/produkte.asp?typ=fulltext&file=UIN2006076001057

Capabilities (immunity) do not evolve - beings do.

What are you talking about? Face wash bacteria? Milk? Spearmans correlation coefficient?

Alot of anthropomorphizing.

Bird flu H5N1risk as a significant pandemic - despite all the scares and hype - appears to be fading as a possibility. WHO stats for 2007 show a clear decline and total deaths in all the years since description approximate only 200 world wide. Is this yet another screw up by WHO/CDC et al.? Will bird flu H5N1 join BSE, SARS, swine flu even Ebola in the pandemic public health scare "never mind" circular file?

You should have some technical rationale - some reason to think this approach has any value. What is the variable here - oxygen tension? If so - do some homework (ala pubmed) - does staph grown under anaerobic or microaerophilic conditions show differential sensitivities vs. the antimicrobial you plan to use? And just what "novel antim icrobial" do you have in m ind?

The factors you should consider are pH, Aw (water activity), temperature.

Each member of our normal flora has the potential to cause infection/disease under the right circumstances - usually some kind of compromise of the host - immune compromise like AIDs, physical compromise such as a wound. Even the wimpy Malassezia. No Septicemia is not just bacteremia (bacteria in the blood stream) - it's that WITH systemic inflammatory response. Not aware "toxins" breakdown is a major function of bacteria in the gut. What toxins do you mean?