I am an undergraduate working on a final project and I need some help if anyone can...
I have run FACS analysis of thymocytes from 508 and 506 TCR tsg Rag -/- mice exposed to the WT 3K peptide and 6 alanine subsitutions (mutated variants) and have found evidence of negative selection occuring (death correlating with the increase in CD69 expression).
I also have data from harvested splenocytes (proliferation assay) that demonstrate positive selection correlating with the increase in CD69 expression. These two things pretty much just demonstrate that the peptide-MHC-TCR conjuction is effective in allowing development to proceed.
However I am supposed to identify patterns in the way the two different TCRs recognize the WT peptide. Certainly some mutations are less effective in 506 than in 508 and vice versa but this seems a bit shallow to just say that. Should I be looking for something else? I can show my data here if necissary. What else can I really derive from this?
What else can I talk about in my results? If anyone can help or have any suggestions please please tell me...
