Odto

Hi, Thanks for this. I'm going to run the experiment using stated volumes but also at two other much lower volumes, one at my 25mL and see if this has any affect on the total lipid extracted from the biomass. I should be able to see from this if the volume has an affect then... then to discover from the literature why! Cheers

Hi, Can anyone help me with this question. Why does the Lewis et al Modified Bligh & Dyer method (and many more) use vast volumes of solvent >80mL for 500mg of sample for? I have read many articles, including the Folch method, I can't get access to the origional Bligh & Dyer unfortunately, wherein may lie the answer. There seems to be no standard rule to the volumes used/sample mass or volume however I have seen many variations. Having discussed with a chemistry expert, I am biologist with interest more specifically at the molecular and biochemsitry level, the volume has been adjust to max of 25ml/500mg sample. But without any clarification. I can't find the answer to my question after fruitless searches apart from an ad hoc mix of differeing volumes used. Is it something to do with equilibriums changing and extraction becoming more efficient with greater volumes? Any help in answering this, or directing to relevant material which may help will be much apprecaited! Odto PS - I am using a simple method which is non-modifying to lipid structure, unlike FAME, the lipids are to be used in subsequent binding assays so must remain in native form.