mahesh_chavan

-Thank you so much fo your replies. Your suggestion for using centrifuge machine is great, because then can use it separate fat from soya milk instead of using de-oiled soya cakes. I have read that patent for crsystalization of amino acids. Your suggestion for using soda by carb or food grade Sodium Hydroxide is good. And offcourse they must be of food grade. But When I read patent, it become clear to me that I can concentrate aq. solution under reduced pressure to obtain amino acid crystals. Even though they contain air bubbles and of small size, they will do. Because I do not need pure crystals of individual amino acid. I can eat a mixture of amino acid. In any case, If I am not using any other chemicals, there is no risk of any poison getting consumed. I shall be adding diastase first to say de-fatted soya milk. That will convert carbohydrates to sugar. Then I shall use centrifuge to to separate that layer after filtration. if I boil that layer diastase will become inactive. Because diastase is cheap they do not try to reclaim it when corn syrup is prepared from corn starch. Simmlarly, I shall be adding papain to the protein part. And then concentrate under reduced pressure to obtain amino acid crystals. Even if papain gets crystalize, I can eat that. Because papain is present in Papaya fruit and the quantity used will be so minute that it can be consumed without any harm if used in such quantity that it can not further be active ( activity is about 80000 u/g ) Thank you so much. Please let me know if there is any flaw in my proposition. I have come across an article to separate sucrose and glucose /fructose from a mixture using animal charcoal. When the the mixture is passed through animal charcoal column mix. of glucose/fructose comes out straight away. Sucrose is adhered to the column which can be washed using ethanol. I do not have sucrose in the solution. But adsorption chromatography can separate sugars from amino acids. I do not want to use animal charcoal. But will the plant charcoal do ? I do not know which will adhere to the column. Any suggestion / clues for this method ?

Currently I am suffering from a liver disease. I can eat diastase + papain tablets for digestion. But when I was doing my B.Sc. we have put one egg in juice of papaya ( containing ) papain. Within 3-4 hours it was dissolved and amino acids were formed in the juice. But how to separate amino acids from the juice ? ( I do not want individual amino acids just a mixture will do ) But papain must be separated for re-use In fact I want to separate carbohydrates and proteins from a food sample. Take an example of soy flour which contains all required amino acids. If I press the beans, oil can be extracted. remaining cake will contain mixture of carbohydrates and proteins. If I use fungal diastase it will convert carbohydrates into sugar ( glucose + fructose) now I want to separate these sugars from protein part and then put this protein part in papain o get amino acids. Again how to separate them from papain . I do not want to use HPLC or other costly equipments. Can I get separate sugars and amino acid using some solvents and doing crystalization ? Again except water any other solvent must be separated and re-used. Please guide how can I achieve this. ( any techniques) Thanking you